Restriction endonuclease

Restriction endonucleases are bacterial enzymes that stick double- stranded DNA. Type I REs are important in bacterial function but don't stick DNA at specific sequences. Type II REs, described for use in this primer, bear largely specific spots for DNA fractionalization and are therefore extremely useful tools in molecular biology. These enzymes allow the cloning and sanctification of defined DNA fractions. The host bacteria's DNA is defended from fractionalization because the specific recognition spots are modified, generally by methylation at one of the bases in the point, making the point no longer a substrate for shaft fractionalization. Virtually, REs with different recognition point particularity have been purified from colorful bacterial strains and are used by molecular biologists under defined conditions to stick purified DNA from eukaryotic sources into defined fractions in an in vitro response.

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