Genetics & Gene Therapy

Gene Vectors

vector itself generally carries a DNA sequence that consists of an insert and a larger sequence that serves as the" backbone" of the vector. The purpose of a vector which transfers inheritable information to another cell is generally to insulate, multiply, or express the insert in the target cell. All vectors may be used for cloning and are thus copying vectors, but there are also vectors designed especially for cloning, while others may be designed specifically for other purposes, similar as recap and protein expression. Vectors designed specifically for the expression of the transgene in the target cell are called expression vectors, and generally have a protagonist sequence that drives expression of the transgene. Simpler vectors called recap vectors are only able of being transcribed but not restated they can be replicated in a target cell but not expressed, unlike expression vectors. Recap vectors are used to amplify their insert.